IMR Press / FBL / Volume 6 / Issue 2 / DOI: 10.2741/ammirat

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Detection of TNF inhibitors (soluble receptors) in the sera and tumor cyst fluid of patients with malignant astrocytomas of the brain
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1 Division of Neurosurgery, Albert Einstein Medical Center, Klein Professional Building, Suite 501, 5401 Old York Road, Philadelphia, PA, USA
2 Marshfield Clinic Neurosurgery, University of California, Irvine, CA
3 Department of Molecular Biology and Biochemistry, University of California, Irvine, CA
Front. Biosci. (Landmark Ed) 2001, 6(2), 17–24;
Published: 1 October 2001

Patients with malignant astrocytomas of the brain exhibit varying degrees of immunosuppression with only a few factors responsible for this immunosuppression having been characterized. The soluble forms of the 55 kDa and 75 kDa membrane receptors for tumor necrosis factor (sTNF-R's) have been shown to bind to and inhibit the activity of TNF. The present studies analyze levels of sTNF-R's in the sera and tumor cyst fluids of patients with malignant astrocytomas. Using sensitive ELISA techniques, serum levels of the 55 and 75 kDa sTNF-R's in 17 patients tested were found to be elevated [55 kDa of 2.29 ± 2.85 ng/ml and 75 kDa of 4.98 ± 4.03 ng/ml] as compared to 20 normal controls [55 kDa of of 1.21 ± 0.91 ng/ml and 75 kDa of 1.85 ± 0.40 ng/ml] although this was only statistically significant for the 75 kDa sTNF-R (P=0.006). Brain tymor cyst fluid samples obtained from eight patients were shown to have very high levels of both sTNF-R's ranging from 4.16 to 17.17 ng/ml for the 55 kDa receptor and 4.83 to 19.96 ng/ml for the 75 kDa receptor. Six of these cyst fluid samples were also tested for their ability to inhibit TNF cytolytic activity using an in vitro assay. All samples tested had TNF inhibitory activity. Immunohistochemical studies on patient tumor samples showed high levels of expression of these receptors both in the cytoplasm and the cell surface of astrocytoma cells.

We propose that sTNF-R's may be shed by astrocytoma cells and may have a role in both local and systemic immunosuppression observed in astrocytoma patients. Finally, the potential role of serum level of sTNF-R's as tumor markers to follow the treatment and the progression of disease in these patients are discussed.

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