IMR Press / FBL / Volume 29 / Issue 5 / DOI: 10.31083/j.fbl2905180
Open Access Original Research
Newly Isolated Limosilactobacillus reuteri B1/1 Modulates the Expression of Cytokines and Antimicrobial Proteins in a Porcine ex Vivo Model
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1 Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy in Košice, 040 01 Košice, Slovakia
2 Center of Clinical and Preclinical Research MediPark, Faculty of Medicine, P.J. Šafárik University in Košice, 040 01 Košice, Slovakia
3 Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, 040 01 Košice, Slovakia
*Correspondence: viera.karaffova@uvlf.sk (Viera Karaffová)
Front. Biosci. (Landmark Ed) 2024, 29(5), 180; https://doi.org/10.31083/j.fbl2905180
Submitted: 25 January 2024 | Revised: 21 March 2024 | Accepted: 8 April 2024 | Published: 10 May 2024
Copyright: © 2024 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Background: The epithelia of the intestine perform various functions, playing a crucial role in providing a physical barrier and an innate immune defense against infections. By generating a “three-dimensional” (3D) model of cell co-cultures using the IPEC-J2 cell line and porcine blood monocyte-derived macrophages (MDMs), we are getting closer to mimicking the porcine intestine ex vivo.Methods: The effect of Limosilactobacillus reuteri B1/1 and Limosilactobacillus fermentum CCM 7158 (indicator strain) on the relative gene expression of interleukins (IL-1β, IL-6, IL-8, IL-18 and IL-10), genes encoding receptors for TLR4 and TLR2, tight junction proteins such as claudin-1 (CLDN1), occludin (OCLN) and important antimicrobial proteins such as lumican (LUM) and olfactomedin-4 (OLMF-4) was monitored in this model. Results: The results obtained from this pilot study point to the immunomodulatory potential of newly isolated L. reuteri B1/1, as it was able to suppress the enhanced pro-inflammatory response to lipopolysaccharide (LPS) challenge in both cell types. L. reuteri B1/1 was even able to up-regulate the mRNA levels of genes encoding antimicrobial proteins LUM and OLFM-4 and to increase tight junction (TJ)-related genes CLDN1 and OCLN, which were significantly down-regulated in LPS-induced IPEC-J2 cells. Conversely, L. fermentum CCM 7158, chosen as an indicator lactic acid bacteria (LAB) strain, increased the mRNA levels of the investigated pro-inflammatory cytokines (IL-18, IL-6, and IL-1β) in MDMs when LPS was simultaneously applied to basally deposited macrophages. Although L. fermentum CCM 7158 induced the production of pro-inflammatory cytokines, synchronous up-regulation of the anti-inflammatory cytokine IL-10 was detected in both LAB strains used in both cell cultures. Conclusions: The obtained results suggest that the recently isolated LAB strain L. reuteri B1/1 has the potential to alleviate epithelial disruption caused by LPS and to influence the production of antimicrobial molecules by enterocytes.

Keywords
co-culture
IPEC-J2
MDM
lactobacilli
tight-junctions
cytokines
Funding
APVV-21-0129/Slovak Research and Development Agency
VEGA 1/0098/22/Scientific Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic
Figures
Fig. 1.
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