†These authors contributed equally.
Background: Although the role of dynamic factors in DNA function still
remains unclear, research in this direction is a rapidly developing area of
molecular biology. In this work, the genetic constructions appY_red and
appY_green, based on the plasmid pPF1 and containing a fragment of
Escherichia coli (E. coli) DNA with predicted promoter-like
regions, are considered complex dynamic systems in which local sites of double
helix unwinding, called open states, can arise and propagate. The purpose of the
article is to show the existence of a connection between the dynamics of open
states and the functioning of predicted promoters. Methods: We
experimentally verified the functionality of the predicted promoters using a
reporter vector. Using a reverse transcription reaction, transcription start
sites were identified indicating the presence of two divergent promoters, one on
each strand. In mathematical studies, a dynamic model was used that described
open states as one-soliton solutions (kinks) of a system of nonlinear partial
differential equations, and the influence of the torque M