- Academic Editor
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†These authors contributed equally.
Background: The role of Pin2 telomeric repeat factor 1-interacting telomerase inhibitor 1 (PinX1) in tumorigenesis and development has been
extensively studied. As we previously demonstrated, PinX1 plays an important role
in modulating epithelial-mesenchymal transition (EMT), stemness, cell
proliferation, and apoptosis in nasopharyngeal carcinoma (NPC). However, the
relationship between PinX1, autophagy, and cell function in NPC remains unclear.
This study aimed to investigate the mechanisms by which PinX1 regulates autophagy
in NPC, and to explore its biological role and clinical significance in disease
progression. Methods: The proliferative capacity of NPC cells was
assessed by MTT and xenograft tumorigenicity assays. Autophagic flux was
monitored using a tandem monomeric DAPI–FITC–LC3 reporter assay. The rates of
apoptosis and the cell cycle in NPC cells were analyzed using flow cytometry. The
activation of autophagy and the signaling status of the AKT/mTOR and
NF-