IMR Press / FBL / Volume 28 / Issue 5 / DOI: 10.31083/j.fbl2805104
Open Access Original Research
TRPM7 is Involved in the Regulation of Proliferation, Migration and Osteogenic Differentiation of Human Dental Follicle Cells
Dongchuan Zuo1,2,†Jiali Li3,4,†Yueyue Huang3,4Jiantao Li3,4Shunzhi Yao3,4Lei Xiong3,4Jin Zeng3,4,*
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1 The Key Laboratory of Medical Electrophysiology, Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, 646000 Luzhou, Sichuan, China
2 Department of Cardiology, The Affiliated Hospital of Southwest Medical University, 646000 Luzhou, Sichuan, China
3 Department of Orthodontics, The Affiliated Stomatology Hospital of Southwest Medical University, 646000 Luzhou, Sichuan, China
4 Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, The Affiliated Stomatological Hospital of Southwest Medical University, 646000 Luzhou, Sichuan, China
*Correspondence: (Jin Zeng)
These authors contributed equally.
Front. Biosci. (Landmark Ed) 2023, 28(5), 104;
Submitted: 1 September 2022 | Revised: 24 November 2022 | Accepted: 29 November 2022 | Published: 25 May 2023
Copyright: © 2023 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: Dental follicle cells (DFCs) are promising candidates for tissue engineering. However, the molecular mechanisms that regulate the biological characteristics of DFCs are still unclear. Transient receptor potential melastatin 7 (TRPM7) is a Ca2+- and Mg2+-permeable cation channel. The aim of this study was to determine the impact of TRPM7 on the proliferation, migration and osteogenic differentiation of DFCs. Methods: PCR, Western blotting, Immunocytochemical staining and Patch clamp methods were used to identify the gene and protein expression of TRPM7 in DFCs. DFCs were infected with lentiviruses that expressed either TRPM7 specific shRNA or scrambled non-effective shRNA to investigate its functional role. Cell proliferation and migration were assessed using Cell Counting Kit-8 assays and transwell cell culture chambers separately. Cell osteogenic differentiation were determined by ALP assay kit and Alizarin Red staining. Results: Gene and protein expression of TRPM7 were detected in DFCs, but not of TRPM6, which is a closely related channel with similar function. In the absence of Mg2+, typical whole cell TRPM7-like currents were recorded by patch clamp. These were inhibited by low concentrations of 2-APB, but activated by high concentrations of 2-APB. Functional studies demonstrated that suppression of TRPM7 expression inhibited the proliferation and migration of DFCs, and promoted their osteogenic differentiation. Furthermore, Mg2+ deficiency mimicked the effects of TRPM7 knockdown in terms of osteogenic differentiation of DFCs. Conclusions: These results demonstrate that TRPM7 is involved in regulating the proliferation, migration and osteogenic differentiation of DFCs.

osteogenic differentiation
2023NSFSC1522/Sichuan Science and Technology Program
2022YFS0634/Sichuan Science and Technology Program
2020LZXNYDJ05/Luzhou-Southwest Medical University Joint Project
2019LZXNYDJ01/Luzhou-Southwest Medical University Joint Project
Fig. 1.
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