- Academic Editor
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†These authors contributed equally.
Background: Lung adenocarcinoma (LUAD) is one of the most
common and lethal cancer types worldwide.
LINC0572 is a long non-coding RNA (lncRNA) that has been associated with the
clinical characteristics of several types of malignancy. However, the biological
mechanism of LINC0572 in LUAD is still unclear and remains to be elucidated.
Methods: R packages and online bioinformatic tools were used to investigate the biological
characteristics of LINC01572, including its abnormal expression, oncogenic role,
and clinical prognostic value. In vitro and in vivo experiments
were conducted to investigate the biological functions of LINC01572 in
tumorigenesis and development. These included colony formation assays, cell
migration assays, flow cytometry, cell counting kit-8 (CCK-8) cell proliferation and tumor transplant
growth experiments.
Results: Bioinformatics
results showed that LINC01572 was overexpressed in both LUAD and lung squamous
cell carcinoma (LUSC) patients. LINC01572 overexpression was associated with
shorter overall survival (OS) in LUAD. Further study of clinical specimens
confirmed that LINC01572 was highly expressed in the tumor tissue of non-small
cell lung cancer (NSCLC) patients. In vitro experiments also confirmed
that LINC01572 was overexpressed in tumor cell lines. Inhibition of LINC01572
expression significantly impaired cell proliferation, cell migration, and clone
formation. Experiments in nude mouse revealed that transplanted tumors with low
expression of LINC01572 had significantly slower rates of growth in terms of
volume and weight compared to the control group (p