IMR Press / FBL / Volume 27 / Issue 9 / DOI: 10.31083/j.fbl2709261
Open Access Original Research
Human Parvovirus B19 Nonstructural Protein 1 Regulates GATA1 Expression via the Notch Signaling Pathway in K562 Cell Line
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1 Department of Pediatrics, Children's Digital Health and Data Center, Zhongnan Hospital of Wuhan University, 430031 Wuhan, Hubei, China
*Correspondence: zhao_wh2004@hotmail.com (Dongchi Zhao)
These authors contributed equally.

Academic Editor: Ioanna-Katerina Aggeli
Front. Biosci. (Landmark Ed) 2022, 27(9), 261; https://doi.org/10.31083/j.fbl2709261
Submitted: 9 May 2022 | Revised: 18 July 2022 | Accepted: 27 July 2022 | Published: 16 September 2022
(This article belongs to the Special Issue Signal Transduction and Gene Expression for Modeling Human Diseases)
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Background: Human parvovirus B19 (B19) infection can affect the hematopoietic arrest in fetus by hindering the differentiation and maturation of erythroid progenitor cells. B19 nonstructural protein 1 (NS1) has been shown to inhibit the differentiation of erythroid progenitor cells. The goal of this study is to explore the role of B19 NS1 in the regulation of GATA1 and Notch signaling pathway in hematopoietic cells. Methods: The B19 NS1 expression plasmid was reconstituted, and the possibility of NS1 regulating GATA1 and GATA2 expression modulated by Notch-Hes pathway was tested by qRT-PCR and western blot. Immunofluorescence assays were conducted to visualize pNS1 in K562 cells. Results: We demonstrate that B19 NS1 inhibited GATA1 and induced Hes1/Hes5, which is involved in the activation of Notch signaling pathway. Meanwhile, NS1 exhibited promoting effects on GATA2 expression. Activation of the Notch signaling pathway up-regulated its downstream transcriptional repressor family Hes, thereby inhibiting the expression of GATA gene in K562 cells. Conclusions: The results show that B19 NS1 protein negatively regulates GATA1 related nuclear transcription and may interfere with hematopoietic cell differentiation.

Keywords
human parvovirus B19
nonstructural protein 1
GATA
Notch
hematopoietic cells
Figures
Fig. 1.
Funding
81170005/ Chinese National Natural Science Fund
81670007/ Chinese National Natural Science Fund
WJ2019M214/ e Scientific Project supported by the Health Commission of Hubei Province, China
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