Fig. 1.ANO1 is up-regulated in mouse and human ADPKD kidneys. (A)
Quantitative PCR analysis of ANO1 expression in kidney tissues of PKD1 mice with different ages (1, 3, 5, 18 months). (B) Quantitative PCR analysis of
ANO1 expression in human normal control and ADPKD patients renal tissues. (C,D)
Western blotting analysis of ANO1 expression in human normal control and ADPKD
patients renal tissues and was further quantified. (E) Immunohistochemistry
analysis of ANO1 (in brown) expression in human normal control (N1, N2, N3) and
ADPKD patients (A1, A2, A3) renal tissues. IgG served as a negative control for
staining. Scale bars, 200 m. On representative result from three
independent experiments was shown. *p 0.05. **p 0.01.