†These authors contributed equally.
Academic Editors: Marcus Franz and Alexander Pfeil
Objective: The aim of this study was to explore the effect of
concentrated growth factor (CGF) on the wound healing potential of human
epidermal cells (HaCaT) in vitro and in vivo. Methods:
CGF was extracted from venous blood using the centrifugal separation method. The
CGF-conditioned medium was prepared from CGF gel immersed in Dulbecco’s Modified
Eagle medium. Crystal violet staining and wound healing assay were used to
evaluate the proliferation and migration of HaCaT cells, respectively.
Lipopolysaccharide (LPS) was used to test the anti-inflammatory function of CGF.
An ELISA kit was employed to detect the concentration of growth factors and
interleukins in CGF medium. mRNA and protein levels of angiogenic biomarkers
(Angiopoietin-1 (ANGPT-1), vascular endothelial growth factor-A (VEGF-A) and Angiopoietin-2 (ANGPT-2) ) were determined by quantitative polymerase chain
reaction (qPCR) and Western blot, respectively. A dorsal excisional wound model
was recruited to test the wound healing effect of CGF in mice. Results:
Three-day treatment of HaCaT cells with CGF significantly promoted cell
proliferation, which was followed by an increase in Vascular Endothelial Growth Factor (VEGF) and Fibroblast Growth Factor (FGF) levels in the
medium. Cytokines (IL-6, IL-8 and TNF-