IMR Press / FBL / Volume 27 / Issue 11 / DOI: 10.31083/j.fbl2711299
Open Access Original Research
Dipsacoside B Inhibits the Migration and Proliferation of VSMCs and Blunts Neointimal Formation by Upregulating PTEN Expression
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1 Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, The School of Pharmacy, Hunan University of Chinese Medicine, 410208 Changsha, Hunan, China
2 Hunan Key laboratory of Vascular Biology and Translational Medicine, Medical School, Hunan University of Chinese Medicine, 410208 Changsha, Hunan, China
*Correspondence: (Qinhui Tuo)
These authors contributed equally.
Academic Editors: Marcus Franz and Alexander Pfeil
Front. Biosci. (Landmark Ed) 2022, 27(11), 299;
Submitted: 5 July 2022 | Revised: 26 September 2022 | Accepted: 27 September 2022 | Published: 3 November 2022
(This article belongs to the Special Issue New Frontiers in Vascular Remodeling)
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: To investigate the effect and potential molecular mechanisms of Dipsacoside B (DB), an herb monomer extracted from Dipsacusasper or Lonicera macranthoides, on the migration and proliferation of vascular smooth muscle cells (VSMCs) and balloon-induced neointimal formation. Methods: In vivo, rat abdominal aorta balloon injury model was utilized to investigate the effect of DB on the neointimal formation. In vitro, cultured VSMCs were used to investigate the effect of DB on Angiotensin-II (Ang-II)-induced migration and proliferation of VSMCs. Western blot and immunofluorescence were used to measure PTEN expression. Results: As compared to vehicle control balloon-injury group, DB treatment significantly inhibited the neointimal formation together up-regulated the expression of phosphatase and tension homolog deleted on chromosome 10 (PTEN). Cell proliferations (MTT and Edu incorporation) assays and wound migration measurement further revealed that treatment with DB significantly blunted Ang-II-induced proliferation and migration potential of VSMCs. Western blot analysis exhibited that DB upregulated the expression of PTEN in vivo and in vitro. Conclusions: DB treatment suppresses the proliferation and migration of VSMCs and reduces neointimal formation by the mechanisms involving regulating the phenotype switch of VSMCs via upregulating PTEN expression.

dipsacoside B
vascular smooth muscle cell
phenotype switch
balloon injury
2022SK2011/Key Research and Development Projects of Hunan Provincial Science and Technology Department
2021RC4064/Science and Technology Innovation Program of Hunan Province
20A379/Key Projects of Hunan Provincial Department of Education
kq2202268/Natural Science Foundation of Changsha
2021CX10/Graduate Innovation Project of Hunan University of Chinese Medicine
2021XJJJ010/Graduate Innovation Project of Hunan University of Chinese Medicine
Fig. 1.
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