Introduction: Tadpole tail develops from the tailbud, an apparently
homogenous mass of cells at the posterior of the embryo. While much progress has
been made in understanding the origin and the induction of the tailbud, the
subsequent outgrowth and differentiation have received much less attention,
particularly with regard to global gene expression changes. Methods: By
using RNA-seq with SMRT and further analyses, we report the transcriptome
profiles at four key stages of tail development, from a small tailbud to the
onset of feeding (S18, S19, S21 and S28) in Microhyla fissipes, an
anuran with a number of advantages for developmental and genetic studies.
Results: We obtained 48,826
transcripts and discovered 8807 differentially expressed transcripts (DETs, q
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Transcriptome profiling reveals gene regulation programs underlying tail development in the Ornamented Pygmy frog Microhyla fissipes
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1
Chengdu Institute of Biology, Chinese Academy of Sciences, 610041 Chengdu, Sichuan, China
2
Section on Molecular Morphogenesis, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH), Bethesda, Maryland, MD 20892, USA
*Correspondence: Shi@helix.nih.gov (Yun-Bo Shi); jiangjp@cib.ac.cn (Jianping Jiang)
Front. Biosci. (Landmark Ed) 2021, 26(11), 1001–1012;
https://doi.org/10.52586/5004
Submitted: 2 July 2021 | Revised: 25 July 2021 | Accepted: 10 August 2021 | Published: 30 November 2021
(This article belongs to the Special Issue Twenty-five years at the frontiers of knowledge: a quarter-century of "Frontiers in Bioscience")
Copyright: © 2021 The Author(s). Published by BRI.
This is an open access article under the CC BY 4.0 license (https://creativecommons.org/licenses/by/4.0/).
Abstract
Keywords
SMRT sequencing
RNA-Seq
Regeneration
Tail development
Microhyla fissipes
Figures
Fig. 1.