We have investigated the anti-oxidative and glucose-lowering effects of 70% methanolic extract of H. heteroclita fruit pulp (MHE). Anti-oxidative property of MHE was assessed by free radical scavenging assays and compound level screening by LC-MS profiling. In silico analysis and in-vivo preclinical validation were also performed using molecular docking and alloxan-induced diabetic model, respectively. MHE showed high anti-oxidant activity in DPPH radical scavenging assay with an IC₅₀ of 0.37 μg/mL. The LC-MS profile of MHE substantiated the presence of p-hydroxy acids including benzoic, salicylic, p-coumaric, o-coumaric, caffeic, protocatechuic, gentisic, 2,4-dihydroxybenzoic, gallic, vanillic, syringic, and ferulic acids. Among these, caffeic acid was the most likely compound to interact with 1R0E at Val⁷⁰, Ala⁸³, Lys⁸⁵, Gly⁹⁷, Asp¹³³, Val¹³⁵, Leu¹⁸⁸, Cys¹⁹⁹, and Asp²⁰⁰, and with 1Q4L at Ile⁶², Ala⁸³, Asp¹³³, Tyr¹³⁴, Val¹³⁵, Arg¹⁴¹, and Val¹⁷⁰. Treatment of rats with MHE showed significant reduction in serum glucose levels as compared to control rats. Taken together, the results show that MHE has compounds with anti-diabetic effect, which could be partially due to the anti-oxidant effects of the active components in MHE.