IMR Press / FBL / Volume 23 / Issue 5 / DOI: 10.2741/4622

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Identification of bioactive glucose-lowering compounds of methanolic extract of Hodgsonia heteroclita fruit pulp

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1 DBT-BIF Facility, BT-Finishing School, Science Research Centre, Department of Biochemistry and Biotechnology, Maharani Lakshmi Ammanni College for Women, Bengaluru-560012, Karnataka, India
2 Centre for Bamboo Studies, Bodoland University, Kokrajhar-783370, Bodoland Territorial Area Districts (BTAD), Assam, India
3 Department of Biotechnology, Bodoland University, Kokrajhar-783370, BTAD, Assam, India
4 Department of Biochemistry, CPGS, Jain University, Jayanagar, Bangalore 560011, India
5 Biotechnology Information Centre, Department of Biotechnology, Govt. of India, New Delhi, India
6 Heymans Institute of Pharmacology, Faculty of Medicine and Health Sciences, Ghent University, Gent, Belgium
Front. Biosci. (Landmark Ed) 2018, 23(5), 875–888; https://doi.org/10.2741/4622
Published: 1 January 2018
(This article belongs to the Special Issue Aging in animals)
Abstract

We have investigated the anti-oxidative and glucose-lowering effects of 70% methanolic extract of H. heteroclita fruit pulp (MHE). Anti-oxidative property of MHE was assessed by free radical scavenging assays and compound level screening by LC-MS profiling. In silico analysis and in-vivo preclinical validation were also performed using molecular docking and alloxan-induced diabetic model, respectively. MHE showed high anti-oxidant activity in DPPH radical scavenging assay with an IC50 of 0.37 μg/mL. The LC-MS profile of MHE substantiated the presence of p-hydroxy acids including benzoic, salicylic, p-coumaric, o-coumaric, caffeic, protocatechuic, gentisic, 2,4-dihydroxybenzoic, gallic, vanillic, syringic, and ferulic acids. Among these, caffeic acid was the most likely compound to interact with 1R0E at Val70, Ala83, Lys85, Gly97, Asp133, Val135, Leu188, Cys199, and Asp200, and with 1Q4L at Ile62, Ala83, Asp133, Tyr134, Val135, Arg141, and Val170. Treatment of rats with MHE showed significant reduction in serum glucose levels as compared to control rats. Taken together, the results show that MHE has compounds with anti-diabetic effect, which could be partially due to the anti-oxidant effects of the active components in MHE.

Keywords
Diabetes
Treatment
Bodo tribe
LC-MS
Hodgsonia heteroclita
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