IMR Press / FBL / Volume 22 / Issue 8 / DOI: 10.2741/4549

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Macrophage migration inhibitory factor siRNA inhibits hepatic metastases of colorectal cancer cells

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1 Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China
2 Department of Gastroenterology, The 101 Hospital of the Chinese People’s Liberation Army, Wuxi, Jiangsu 214000, China
3 Department of Gastroenterology, Qingyuan Renmin Hospital, Qingyuan, Guangdong 511500, China
4 Institute of Vascular Biology, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China

Academic Editor: Pin-Lan Li

Front. Biosci. (Landmark Ed) 2017, 22(8), 1365–1378; https://doi.org/10.2741/4549
Published: 1 March 2017
(This article belongs to the Special Issue Molecular pathobiology of lipid mediators)
Abstract

The purpose of this study was to assess the anti-tumor effects of macrophage migration inhibitory factor (MIF) siRNA on colorectal cancer in a mouse xenograft model. MIF specific siRNA (MIF siRNA) or a nonspecific control siRNA was introduced to murine colorectal cancer CT-26 cells. Mouse xenograft models of colorectal cancer were established. MIF siRNA, control siRNA or water was injected twice a week intravenously for 4 weeks. MIF siRNA inhibited the proliferation and migration, while induced apoptosis of CT-26 cells in vitro. Injection of MIF siRNA resulted in a significant decrease of serum MIF and VEGF levels, and the weight and volume of cecum-grafted tumors in vivo. In contrast, the number of apoptotic cells and caspase-3 expression were increased by MIF siRNA in cecum graft tumor tissues. Moreover, the water and fodder consumption were significantly improved by MIF siRNA treatment. Importantly, MIF siRNA reduced the hepatic metastases from colorectal cancer. Our results suggest that siRNA targeting MIF is a promising agent for the treatment of hepatic metastasis of colorectal cancer cells.

Keywords
Colorectal cancer
Hepatic metastases
Macrophage migration inhibitory factor
SiRNA
Tumor suppressor
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