IMR Press / FBL / Volume 16 / Issue 7 / DOI: 10.2741/3870

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Experimental study of 99mTc-depreotide preparation and its affinity with A549 cell

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1 Department of Nuclear Medicine, 10th People’s Hospital, Tongji University of Medcine School, Shanghai 200072, China
2 Department of Nuclear Medicine, Shanghai Putuo Distict People’s Hsopital, Shanghai 200060, China
3 Department of Radiology, Tongji Hospital,Tongji University,Shanghai 200071, China
Academic Editor:Wei Qin
Front. Biosci. (Landmark Ed) 2011, 16(7), 2527–2539; https://doi.org/10.2741/3870
Published: 1 June 2011
(This article belongs to the Special Issue Pathogenesis and diagnostic modalities in cancer)
Abstract

The 99mTc-labeled agent, [99mTcO]depreotide, has received regulatory approval in the United States and Europe for use in the detection of cancer. It is essential to establish a simple and reliable method of direct radiolabeling of 99mTc-depreotide and to investigate its specific receptor binding properties with human non small cell lung cancer (NSCLC) A549 cell in vitro. So we made some researches as follow: Depreotide was labeled with 99mTc using SnCl2 as a reductant. Labeling efficiencies at different pH values and temperatures were compared. Radioreceptor assay was used to observe the uptake kinetics, stagnation and retention half time of 99mTc-depreotide in A549 cells. As the results of the investigation ,many facts is shown below: The labeling rate of pH 6.0 group was higher than that of pH 5.0 and pH7.0 groups. The labeling rate decreased when temperature increased from 15 °C to 50 °C. The uptake rate increased with rising temperature, and the maximum uptake was observed at 60min at 37 °C. The cleaning curves were similar at different temperatures, and the half cleaning time at 37 °C was 48 min. The results showed that the optimal conditions for labeling depreotide with 99mTc was found to be below 15 °C at a pH lower than 6.0. Furthermore, at 37 °C, 99mTc-depreotid may have the potential as an ideal imaging agent for somatostatin receptors.

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