IMR Press / FBL / Volume 13 / Issue 17 / DOI: 10.2741/3168

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Overexpression of hypoxia/inflammatory markers in atherosclerotic carotid plaques
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1 Department of Neurology, Stroke Unit, University Hospital of Bellvitge (HUB) and IDIBELL, Barcelona, Spain
2 Cardiovascular Research Center, IIBB/CSIC-HSCSP-UAB, Barcelona, Spain
3 Department of Vascular Surgery, University Hospital of Bellvitge (HUB), Barcelona, Spain
4 School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, UK

*Author to whom correspondence should be addressed.


Front. Biosci. (Landmark Ed) 2008, 13(17), 6483–6490;
Published: 1 May 2008

Hypoxia, angiogenesis and inflammation leads to plaque progression and remodelling and may significantly contribute towards plaque rupture and subsequent cerebrovascular events. Our aim was to study, markers of hypoxia and inflammation previously identified by microarray analysis, in atherosclerotic carotid arteries with low to moderate stenosis. We hoped to describe different cellular populations expressing the studied markers. The location of selected inflammatory molecules obtained as vascular transplants from organ donors were analysed by immunohistochemistry with monoclonal and polyclonal antibodies. Paraffin-embedded sections were cut and probed with antibodies recognizing active B and T-lymphocytes (CD30), hypoxia-inducible factor-1alpha, endoglin (CD105), Interleukin-6 and C-reactive protein. We observed a notable overexpression of HIF-1alpha in inflammatory and hypoxic areas of carotid arteries in all types of lesions from type II-V taken from the patients with carotid stenosis <50%. This suggests that HIF-1α may have a putative role in atherosclerosis progression and angiogenesis. Dynamic changes in the non-occluding plaques may explain some of the clinical events in patients with low to moderate carotid stenosis.

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