The most prevalent form of adulteration found in milk and dairy products involves the addition of cow milk to goat milk and dairy products. Detecting such adulteration is crucial in order to prevent health issues, particularly allergies, and to safeguard consumers against financial losses. This research aimed to examine the extent of cow and goat milk mixing at various percentages: 100%, 90%, 75%, 25%, 10%, 5%, 1%, and 0%. To accomplish this, fresh cheese samples were experimentally produced using these milk mixtures, and the levels of cow milk percentage and cow DNA content were determined using TaqMan real-time PCR. The results indicated that the presence of cow milk mixed with goat milk at concentrations as low as 1% and with cow DNA levels of 0.01 ng could be detected in cheese samples. In conclusion, TaqMan-based real-time PCR demonstrates high sensitivity and can be regarded as a reliable method for identifying the presence and proportions of cow milk in cheese samples obtained through the blending of cow and goat milk, thereby ensuring protection against economically driven adulteration and promoting consumer safety.