Die Pharmazie is published by IMR Press from Volume 81 Issue 1 (2026). Previous articles were published by another publisher under the CC-BY licence, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement.
Enantiomeric separation of β2-agonists on macrocyclic antibiotic chiral stationary phases in high performance liquid chromatography
Dandan Zhang 1, Maosheng Cheng 2, Myung Ho Hyun 3, Zhili Xiong 4, Li Pan 2, Famei Li 4
Affiliations
Article Info
1 Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang, 110016, China, Email: dandanzhang625@hotmail.com
2 Department of Medicinal Chemistry, Shenyang Pharmaceutical University, Shenyang, China
3 Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Pusan, Korea
4 Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang, China
Abstract
The enantiomeric resolution of six β2-agonists including bambuterol, clenbuterol, clenproperol, fumoterol, mabuterol and terbutaline, was studied on three macrocyclic antibiotic chiral stationary phases (CSPs): Chirobiotic V, T and R. By varying the mobile phase compositions for different CSPs, all the six compounds were successfully separated on Chirobiotic V and T CSPs. The thermodynamic parameter variation was calculated. The chiral recognition mechanism was discussed, the ionic interaction was confirmed to be the most dramatic interaction responsible for the chiral recognition. No enantiosepartion for all the β2-agonists was found on Chirobiotic R CSP.