International Journal of Pharmacology (IJP) is published by IMR Press from Volume 21 Issue 4 (2025). Previous articles were published by another publisher under the CC-BY licence, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement.
Anti-inflammatory Activities for the Extracts and Carpinontriols from Branches of Carpinus turczaninowii
1 Department of Chemistry and Center for Cosmetic Sciences, Jeju National University, Ara-1, Jeju 690-756, Korea
2 LINC Agency, Jeju National University, Ara-1, Jeju 690-756, Korea
3 Citrus Research Station, National Institute of Horticulture and Herbal Science, Seogwipo 697-943, Korea
Abstract
Development of natural products possessing anti-inflammatory activities has been the focus of research in our laboratory. In this study, the extract from Carpinus turczaninowii branches was studied and their anti-inflammatory constituents were identified. The aqueous ethanol extract was fractionated successively to afford n-hexane, ethyl acetate, n-butanol and aqueous layers. Upon anti-inflammatory tests for Nitric Oxide (NO) production, using RAW 264.7 cells, the ethyl acetate fraction exhibited potent inhibitory activities without causing cell toxicities. The ethyl acetate fraction was subjected to further purification to isolate carpinontriol A and carpinontriol B as active constituents. These diarylheptanoid compounds were isolated for the first time from this plant. The isolates reduced the lipopolysaccharide (LPS)-induced secretion of NO and IL-6 productions in a dose-dependent manner in RAW 264.7 cells which indicates their anti-inflammatory effects. Based on these results, it was suggested that C. turczaninowii extracts containing carpinontriols A and B could be considered potential anti-inflammatory agents for pharmaceuticals or cosmetics.
Keywords
- Carpinus turczaninowii
- carpinontriol A
- carpinontriol B
- anti-inflammation