- Academic Editor
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Objective: This investigation aimed to increase the bioavailability and
anticancer effects of allicin (AC) by encapsulating it in solid lipid
nanoparticles (SLN) decorated with chitosan (CS)-conjugated folic acid (FA).
Material and Methods: Nanoparticles (NPs) were synthesized by
high-pressure homogenization, and then, Fourier-transform infrared (FTIR),
Field-Emission Scanning Electron Microscopy (FESEM), Dynamic Light Scattering
(DLS), and zeta potential methods were used to determine their physicochemical
characteristics. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
(MTT) assay was performed to assess the effect of toxicity and flow cytometry,
while fluorescent staining methods were used to investigate the type of cell
death. Real-time quantitative polymerase chain reaction (qPCR) was used to evaluate the expression levels of apoptotic
genes: Bcl-2, and caspase-8. Results: The presence of
AC-SLN-CS-FA with a spherical morphology, an average size of 86.7