IMR Press / FBL / Volume 27 / Issue 8 / DOI: 10.31083/j.fbl2708248
Open Access Short Communication
OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa
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1 Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, 225009 Yangzhou, Jiangsu, China
2 Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009 Yangzhou, Jiangsu, China
*Correspondence: 006949@ yzu.edu.cn (Yunfei Wu)
Academic Editor: Marcello Iriti
Front. Biosci. (Landmark Ed) 2022, 27(8), 248; https://doi.org/10.31083/j.fbl2708248
Submitted: 17 March 2022 | Revised: 28 April 2022 | Accepted: 2 June 2022 | Published: 17 August 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Background: Crown root is the main part of root system, which performs an important role in rice growth and development, especially in nutrition and water assimilation. Previously, we reported negative feedback regulation loop between Oryza sativa DNA BINDING WITH ONE FINGER 11 (OsDOF11) and cytokinin by Oryza sativa CYTOKININ OXIDASE/DEHYDROGENASE 4 (OsCKX4) in rice development. Methods: Reverse transcription quantitative RT-PCR analyses was used to analyze the related gene transcript level. Nitrogen and hormone were measured by CHN-Nitrogen analyser and Liquid chromatography mass spectrometer, respectively. Exogenous application of cytokinin and [13C] sucrose labeled stable isotope uptake experiments help us to explain the relationship between OsDOF11 and cytokinin. Results: We demonstrate the role of OsDOF11 in root development. We note that the loss function of OsDOF11 displays the reduced crown roots number, low activity of nitrogen assimilation and low content of cytokinin and auxin. The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines). Through Exogenous application of multiple concentrations of cytokinin as treatment to osdof11-1 mutant, RNAi-9 lines, and wild type (WT). We found that the crown roots number of osdof11-1 plants were rescued as the cytokinin concentration increased gradually from 1 μM to 10 μM, but the effect was weaker in RNAi-9 line. And cytokinin inhibited sucrose uptake activity from Murashige-Skoog medium with 3.0% sucrose (MS30) by OsDOF11 in rice root. Conclusions: OsDOF11 promotes crown root formation via cytokinin in oryza sativa. These results provide a physiological basis for further analysis of the OsDOF11 function of in rice root development.

Keywords
OsDOF11
cytokinin
nitrogen
crown root
rice
Figures
Fig. 1.
Funding
Grant BK20190889/ Natural Science Foundation of Jiangsu Province
19KJB180033/ Natural Science Foundation of the Jiangsu Higher Education Institutions of China
2019M660130/ Project funded by China Postdoctoral Science Foundation
202111117039Z/ Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the Undergraduate Research & Practice innovation program of Jiangsu Province
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