IMR Press / FBL / Volume 27 / Issue 7 / DOI: 10.31083/j.fbl2707210
Open Access Original Research
Tumor-Suppressor Gene Transmembrane Protein 98 Promotes Proliferation and Inhibits Apoptosis in Ovarian Cancer
San-Gang Wu1,†Jing-Ying Xu2,†Jian Lei2,†Min Hu2Juan Zhou2,*
Show Less
1 Department of Radiation Oncology, Xiamen Cancer Center, Xiamen Key Laboratory of Radiation Oncology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, 361003 Xiamen, Fujian, China
2 Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, 361003 Xiamen, Fujian, China
*Correspondence: zhoujuan@xmu.edu.cn (Juan Zhou)
These authors contributed equally.
Academic Editors: Viviana di Giacomo and Silvia Sancilio
Front. Biosci. (Landmark Ed) 2022, 27(7), 210; https://doi.org/10.31083/j.fbl2707210
Submitted: 30 March 2022 | Revised: 16 June 2022 | Accepted: 16 June 2022 | Published: 30 June 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Background: Ovarian cancer (OC) is the most deadly tumor in gynecology and there is no effective biomarker for diagnosis and treatment. The role of Transmembrane Protein 98 (TMEM98) in ovarian cancer is still unclear. Methods: The expression and prognostic effect of TMEM98 in OC were analyzed using the public database. Cell Counting Kit-8 proliferation experiment, scratch experiment, Transwell invasion experiment, flow cytometry, TUNEL staining, and in vivo and vitro experiment were used. Results: TMEM98 was significantly downregulated in OC tissues and cell lines compared to the normal ovarian tissue and cells lines. In addition, patients with lower TMEM98 levels exhibited inferior survival. Low expression of the TMEM98 promoted proliferation, migration, invasion, vasculogenic mimicry, and inhibited apoptosis in OC cells. The expression of Caspase-3 was significantly downregulated and the expression of Bcl-2 was significantly increased in the silencing-TMEM98 group. Moreover, low expression of TMEM98 promotes OC development in vivo. Bioinformatics analysis showed that TMEM98 expression was negatively correlated with poly ADP-ribose polymerase expression. Conclusions: This study demonstrates that TMEM98 is low expressed in OC and impacts the prognosis of OC patients. TMEM98 inhibits proliferation and promotes apoptosis and finally exerts a certain tumor-suppressor effect on OC.

Keywords
TMEM98
ovarian cancer
tumor suppressor
prognosis
Figures
Fig. 1.
Funding
2019-ZQNB25/Commission Young and Middle-aged Talents Training Project of Fujian Health Commission
2021GGB027/Commission Young and Middle-aged Talents Training Project of Fujian Health Commission
2020J011240/Natural Science Foundation of Fujian Province
Share
Back to top