IMR Press / FBL / Volume 27 / Issue 5 / DOI: 10.31083/j.fbl2705156
Open Access Original Research
Characterization of the Extracellular Volatile Metabolome of Pseudomonas Aeruginosa Applying an in vitro Biofilm Model under Cystic Fibrosis-Like Conditions
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1 Applied Analytical Chemistry, University of Duisburg-Essen, 45141 Essen, Germany
2 Environmental Microbiology and Biotechnology, University of Duisburg-Essen, 45141 Essen, Germany
3 Instrumental Analytical Chemistry, University of Duisburg-Essen, 45141 Essen, Germany
*Correspondence: (Ursula Telgheder); (Oliver J. Schmitz)
Academic Editors: Massimo Conese and Lorenzo Guerra
Front. Biosci. (Landmark Ed) 2022, 27(5), 156;
Submitted: 12 January 2022 | Revised: 24 March 2022 | Accepted: 24 March 2022 | Published: 13 May 2022
(This article belongs to the Special Issue Cystic fibrosis lung disease: from basic research to clinical issues)
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: Cystic fibrosis (CF) is an autosomal recessive hereditary disease that leads to the production of thickened mucus in the lungs, favouring polymicrobial infections, such as chronic lung infections with the bacterial opportunistic pathogen Pseudomonas aeruginosa. Method: A biofilm model in combination with an adapted sampling and GC-MS analysis method were applied to in vitro studies on different variables influencing the composition of the extracellular volatile metabolome of P. aeruginosa. Results: A significant influence on the metabolome could be demonstrated for the culture medium as well as the atmosphere during cultivation (aerobic or anaerobic). Furthermore, a significant influence of the mucoid (alginate-overproducing) phenotype of the bacterium on quantity and composition of volatile organic compounds could be observed. Based on the results a solid culture medium was developed to simulate the nutrient conditions in the lungs of a CF patient. The extracellular volatile metabolome of bacterial strains P. aeruginosa ATCC 10145, PAO1 and FRD1 was characterized under CF-like conditions. Conclusions: Bacterial strain-dependent metabolites were identified. When P. aeruginosa PAO1 and FRD1 clinical isolates were compared, 36 metabolites showed significant variations in intensities. When the clinical isolates were compared with the reference strain (P. aeruginosa ATCC 10145), 28 metabolites (P. aeruginosa PAO1) and 70 metabolites (P. aeruginosa FRD1) were determined whose peaks showed significant deviation (p > 95%) in intensity. Furthermore, the bacterial strains could be differentiated from each other by means of two principal components.

Pseudomonas aeruginosa
volatile organic compounds
thin-film microextraction
thermal desorption gas chromatography-mass spectrometry
cystic fibrosis
Fig. 1.
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