Academic Editor: Josef Jampilek
Background: Mitochondrial biogenesis occurs in response to chronic
stresses as an adaptation to the increased energy demands and often renders cells
more refractive to subsequent injuries which is referred to as preconditioning.
This phenomenon is observed in several non-neuronal cell types, but it is not yet
fully established in neurons, although it is fundamentally important for
neuroprotection and could be exploited for therapeutic purposes.
Methods: This study was designed to examine whether the preconditioning
treatment with hypoxia or nitric oxide could trigger biogenesis in
undifferentiated and differentiated neuronal cells (rat PC12 and human NT2 cells)
as well as in primary mouse cortical neurons. Results: The results
showed that both preconditioning paradigms induced mitochondrial biogenesis in
undifferentiated cell lines, as indicated by an increase of mitochondrial mass
(measured by flow cytometry of NAO fluorescence) and increased expression of
genes required for mitochondrial biogenesis (Nrf1,
Nrf2, Tfam, Nf