IMR Press / FBL / Volume 27 / Issue 2 / DOI: 10.31083/j.fbl2702062
Open Access Original Research
Effect of Low-Level Er: YAG (2940 nm) laser irradiation on the photobiomodulation of mitogen-activated protein kinase cellular signaling pathway of rodent cementoblasts
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1 AALZ–Aachen Dental Laser Center, RWTH Aachen University, 52074 Aachen, Germany
2 Department of Orthodontics, Faculty of Medicine, Justus Liebig University Giessen, D-35392 Giessen, Germany
3 Department of VIP center, School and Hospital of Stomatology, Cheeloo College of medicine, Shandong University, 250012 Jinan, Shandong, China
4 Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 250012 Jinan, Shandong, China
5 Department of Conservative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, 52074 Aachen, Germany
6 School of Dentistry, Ibirapuera University, 04661-100 Sao Paulo, Brazil
*Correspondence: Jiawen.Yong@dentist.med.uni-giessen.de; jiawen.yong@rwth-aachen.de (Jiawen Yong)
These authors contributed equally.
Academic Editor: Graham Pawelec
Front. Biosci. (Landmark Ed) 2022, 27(2), 62; https://doi.org/10.31083/j.fbl2702062
Submitted: 10 December 2021 | Revised: 5 January 2022 | Accepted: 17 January 2022 | Published: 14 February 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Backgrounds: Dental avulsion due to trauma, especially in young patients, is a worldwide problem, requiring tooth replacement. Delayed replantation could cause tooth loss when the cementum is severely damaged. A small number of studies has reported that photobiomodulation (PBM) therapy using Er: YAG laser irradiation activates cellular signaling responses in different cell types, resulting in a variety of favorable biological effects. The aim of this in vitro study was to evaluate the potential biostimulatory effect of low-level Er: YAG laser irradiation on the biological responses of cultured mouse cementoblasts (OCCM-30), including the mitogen-activated protein kinases (MAPKs). Methods: OCCM-30 cells were exposed to 2940 nm Er: YAG laser irradiation for 15 s at 0.34 W (pulse duration of 100 or 1000 μs, 17 mJ/pulse) at energy densities of 1 or 2 J/cm2. Irradiated and non-irradiated OCCM-30 cells were tested for migration (Scratch assay), proliferation (MTS assay) and functional differentiation (Alizarin Red S assay). Lumican (Lum) and Fibromodulin (Fmod) gene expression, and activation of MAPKs, were assessed by RT-PCR and Western blotting, respectively. Results: Low-level Er: YAG laser irradiation at 2 J/cm2 and pulse duration of 1000 μs resulted in the highest migration rate and proliferation. Moreover, the pulse duration irradiation of 100 μs increased Lum expression. Fmod expression was increased after 1000 μs pulse duration laser stimulation. Low-level Er: YAG laser irradiation increased the mineralization of OCCM-30 cells after 7 days and activated ERK1/2, P38 and JNK signaling. Conclusions: Low-level Er: YAG laser irradiation induces OCCM-30 cell migration, proliferation and differentiation, and activates the MAPK signaling pathway.

Keywords
Er: YAG laser
Cementoblasts
Migration
Proliferation
Cementogenesis
MAPK
Figures
Fig. 1.
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