IMR Press / FBL / Volume 25 / Issue 4 / DOI: 10.2741/4828

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

cPKCβII is significant to hypoxic preconditioning in mice cerebrum

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1 Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
2 Department of Neurology, Hainan Provincial General Hospital, Haikou 570311, China
3 Department of Bioninformatics, School of Basic Medical Sciences and School of Biomedical Engineering, Capital Medical University, Beijing 100069, China
4 Department of Pharmacology, School of Basic Medical Sciences and School of Biomedical Engineering, Capital Medical University, Beijing 100069, China
Front. Biosci. (Landmark Ed) 2020, 25(4), 683–698; https://doi.org/10.2741/4828
Published: 1 January 2020
(This article belongs to the Special Issue Leader sequences of coronavirus are altered during infection)
Abstract

Stroke causes significant morbidity and mortality worldwide, for which no satisfactory preventive option currently exists. Hypoxic preconditioning (HPC) is a protective strategy for cerebral ischemic stroke. To this end, we have identified, Conventional protein kinase C (cPKC)βII to play an important role in HPC. Pathway analysis and protein–protein interaction network building and functional proteomic exploration was used to identify 38 proteins in 6 Kyoto Encyclopedia of Genes and Genomes pathways that interact with cPKCβII in brains subjected to HPC. The role of the oxidative phosphorylation pathway was confirmed by experimental validation, and the demonstration that the activity of the complex I and complex V and expression and activity of Ndufv2 and ATP5d was increased. Ndufv2 was co-localized with PKCβII in neurons rather than glial cells. Together, these data show that Ndufv2 and the oxidative phosphorylation pathway play an important role in cPKCβII-related HPC mediated signalling, likely as an adaptive neuroprotective mechanism.

Keywords
HPC
Proteomic
OXPHO pathway
PPI network
Cellular Signal Transduction
cPKCβII
Figures
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