IMR Press / FBL / Volume 23 / Issue 10 / DOI: 10.2741/4683

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Review

Spheroids of stem cells as endochondral templates for improved bone engineering

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1 Laboratory of Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), Duque de Caxias, Rio de Janeiro, Brazil
2 Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), Duque de Caxias, Rio de Janeiro, Brazil
3 Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio University - Campus I, Brazil
4 Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerem, Duque de Caxias, Rio de Janeiro, Brazil
5 Laboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niteroi, Brazil
Front. Biosci. (Landmark Ed) 2018, 23(10), 1969–1986; https://doi.org/10.2741/4683
Published: 1 June 2018
Abstract

Osteodegenerative disease and bone fractures lead to bone damage or loss, requiring new bone formation to replace the damaged tissues. Classical ‘top-down’ tissue engineering relies on seeding cell suspensions into biomaterial scaffolds, and then guiding cell fate by growth factors. However, complex tissue fabrication using this approach has important limitations. ‘Bottom-up’ tissue engineering has the potential to overcome the drawbacks of the top-down approach, by using ‘building blocks’ of cell spheroids for tissue biofabrication without a scaffold. Spheroids are 3D structures that resemble the physiological tissue microenvironment and can be produced in vitro by different methods. Spheroids of mesenchymal stem cells (MSC) and adipose stem cells (ASC) have regenerative properties. Here we review, the use of spheroids as ‘building blocks’ in the 3D bioprinting of large-scale bone tissue and as a promising alternative for the treatment of osteodegenerative diseases and in bone engineering, including endochondral ossification (or developmental engineering).

Keywords
Bone Engineering
Endochondral Ossification
Spheroids
Biofabrication
Review
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