IMR Press / FBL / Volume 20 / Issue 1 / DOI: 10.2741/4302

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Isoflurane attenuates LPS-induced acute lung injury by targeting miR-155-HIF1-alpha
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1 Department of Anesthesiology, Shanghai Ninth People’s Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, No. 639, Zhi Zao Ju Road, Shanghai, China
2 Central Laboratory, Shanghai Chest Hospital affiliated to Shanghai Jiao Tong University, Shanghai, China
Academic Editor:Fei He
Front. Biosci. (Landmark Ed) 2015, 20(1), 139–156; https://doi.org/10.2741/4302
Published: 1 January 2015
(This article belongs to the Special Issue Cellular immunology and stem cell biology)
Abstract

Isoflurane alleviates the inflammatory response in endotoxin-induced acute lung injury (ALI). In this study, we investigated the protective mechanism of isoflurane postconditioning in lipopolysaccharide (LPS)-induced ALI. Exposure to isoflurane decreased miR-155 and upregulated HIF-1 alpha and HO-1 mRNA and protein. The effects of isoflurane on HIF-1 alpha mRNA and protein could be inhibited by overexpression of miR-155. Furthermore, mice overexpressing miR-155 had higher levels of TNF-alpha and IL-1 beta in BALF when exposed to isoflurane after LPS challenge. Conversely, downregulation of miR-155 promoted isoflurane effects on HIF-1 alpha expression. These results suggest that isoflurane posttreatment at 1 MAC for 4 hour alleviates LPS-induced ALI and cell injury by triggering miR-155-HIF-1 alpha pathway, leading to upregulation of HO-1.

Keywords
Isoflurane
Posttreatment
Endotoxin
Acute Lung Injury
miR-155
HIF1 alpha
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