IMR Press / FBL / Volume 19 / Issue 3 / DOI: 10.2741/4222

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
L-asparaginase induces in AML U937 cells apoptosis via an AIF-mediated mechanism
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1 Department of Hematology/Oncology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical College,Guangzhou, 510623, China
2 Department of Hematology/Oncology, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, 200127, China
3 Department of , Children’s Hospital of Shanghai, Shanghai Jiao Tong University, 200040, Shanghai, China
4 Research Institute of Peadiatric Hemotology, The First Affiliated Hospital of Guangzhou Medical College, 510120, Guangzhou, China
Academic Editor:Wei Qin
Front. Biosci. (Landmark Ed) 2014, 19(3), 515–527; https://doi.org/10.2741/4222
Published: 1 January 2014
(This article belongs to the Special Issue Pathogenesis and diagnostic modalities in cancer)
Abstract

Acute Myeloid Leukemia (AML), a cancer of the myeloid line of blood cells, progresses rapidly and is typically fatal within weeks or months if left untreated. Asparaginases are a class of enzymatic anti-leukemia agents that induce apoptosis in leukemia cell lines; however, the role of L-asparaginase in the induction of apoptosis in AML cells has not been investigated. In this study, we investigated the apoptosis-inducing effect of L-asparaginase and its underlying mechanism in AML U937 cells. The results showed that L-asparaginase significantly inhibited the proliferation of U937 cells by inducing apoptosis. Furthermore, the low baseline expression level of asparaginase synthase (ASNS) demonstrated the sensitivity of U937 cells and AML M5, a rare subtype of AML, to L-asparaginase. Apoptosis induced by L-asparaginase is mediated by apoptosis-inducing factor (AIF). Our findings show the potential of L-asparaginase as an effective approach in treating AML via the induction of apoptosis mediated by AIF.

Keywords
L-asparaginase
Acute Myelomonocytic Leukemia
Apoptosis
ASNS
AIF
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