IMR Press / FBL / Volume 18 / Issue 3 / DOI: 10.2741/4163

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Histone acetylation regulates osteodifferentiation of hDPSCs via DSPP

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1 Department of Endodontics and Operative Dentistry, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China and Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
Academic Editor:Jie Zou
Front. Biosci. (Landmark Ed) 2013, 18(3), 1072–1079; https://doi.org/10.2741/4163
Published: 1 June 2013
(This article belongs to the Special Issue Immunoregulation and inflammatory disease)
Abstract

Dental pulp stem cells (DPSCs) are a unique population of precursor cells isolated from postnatal human dental pulp, with the ability to regenerate a reparative dentin-like complex. We examined the regulation of odontoblast-like differentiation of DPSCs by histone acetylation. Western blot analysis showed that histone H3 acetylation was strongly induced in osteodifferentiation medium. Inhibition of histone acetyltransferase by garcinol reversed osteodifferentiation and mineral formation. Realtime polymerase chain reaction assay indicated that the dentin sialophosphoprotein (DSPP) gene, which is mainly expressed in odontoblasts and preameloblasts in teeth and plays an important role in tooth function, was also downregulated in garcinol-treated cells. Moreover, lentivirusmediated knockdown of DSPP in human DPSCs was associated with significant inhibition of mineral formation, but not osteoblast differentiation. In conclusion, the results of this study suggest that DSPP positively affects mineral formation, and that odontoblast-like differentiation and maturation of DPSCs can be regulated by histone acetylation of the DSPP gene.

Keywords
DSPP
Acetylation Modification
Dental Pulp Stem Cell
Osteo-Differetiation
Mineralization
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