IMR Press / FBL / Volume 13 / Issue 7 / DOI: 10.2741/2857

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Article
Multiple splicing results in at least two p203 proteins that are expressed in the liver and down-regulated during liver regeneration
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1 Life Sciences College, Shandong University, Jinan, Shandong 250100, PR China
2 Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520

*Author to whom correspondence should be addressed.

 

Front. Biosci. (Landmark Ed) 2008, 13(7), 2444–2451; https://doi.org/10.2741/2857
Published: 1 January 2008
Abstract

The interferon inducible p200 family proteins are expressed in a variety of cells and tissues. Intensive studies showed that they were involved in the regulation of cell proliferation and differentiation based on their ability to bind and modulate the activities of multiple transcription activators and inhibitors. Among p200 proteins, p203 has received the least attention and its function is unknown. In the present study, four multiple splicing isoforms of Ifi203, named temporally as Ifi203a/b-1, Ifi203a/b-2, Ifi203 a/b-3, and Ifi203a/b-4, were cloned from the interferon induced 10T1/2 cells. Anti-p203 antiserum was prepared and it could immunodetect a 46 kD and a 51 kD p203 proteins in a variety of cell lines. Unlike other p200 proteins, p203 was exclusively expressed in the liver in the adult C129/SvJ and C57BL/6 inbred strain mice. During the liver regeneration following a standard partial hepatectomy in C57BL/6 mice, the level of p203 decreased significantly in 6-24 h post-operation prior to the cell cycle progression through the G1/S transition.

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