IMR Press / FBL / Volume 12 / Issue 11 / DOI: 10.2741/2387

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Article
Recombinant adeno-associated virus vector hybrids efficiently target different skeletal cells
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1 The Biomedical and Tissue Engineering Group, Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Hong Kong SAR
Front. Biosci. (Landmark Ed) 2007, 12(11), 4280–4287; https://doi.org/10.2741/2387
Published: 1 May 2007
Abstract

Finding the optimal recombinant adeno-associated virus (rAAV) serotypes for efficient as well as tissue specific transduction has become imperative for successful gene therapy. We used rat condylar chondrocytes, osteoblast-like cell line UMR106 and bone marrow stromal cells (BMSCs) to evaluate the transduction efficiency of different rAAV serotypes in vitro; hoping to establish an efficient in vivo rAAV mediated delivery system for gene therapy in craniofacial region. All of the selected rAAV serotypes were able to infect target cells and gave rise to eGFP expression and VEGF secretion. Quantified by fluorescence activated cell sorter (FACS) and ELISA analysis, rAAV2 was superior for efficient transduction of rat chondrocytes, rAAV1 was most efficient when introduced into UMR 106 cell line and rAAV5 yielded the highest infection efficiency in BMSCs. Hence, differences in receptor binding in different oral tissues and transduction pathways suggest rAAV based hybrids have various transduction efficiencies and can efficiently target different oral tissues.

Keywords
AAV
eGFP
VEGF
Chondrocyte
UMR106
BMSC
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