IMR Press / FBL / Volume 11 / Issue 1 / DOI: 10.2741/1784

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Article
Construction and direct immunization of eukaryotic expression plasmid encoding human soluble B lymphocyte stimulator
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1 Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, Beijing, China
2 Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China
Front. Biosci. (Landmark Ed) 2006, 11(1), 121–125; https://doi.org/10.2741/1784
Published: 1 January 2006
Abstract

B lymphocyte stimulator of the tumor-necrosis-factor family (BLyS) enhances B cell survival, a function indispensable to B cell maturation. This factor plays a crucial role in enhancing immune responses. Here, to study the primary immune effect of BLyS gene in animals, we constructed recombinant eukaryotic expression plasmid of the human soluble BLyS by using expression vector pcDNA3.1 (-). The recombinant plasmid pcDNA3.1BLyS was injected subcutaneously into BALb/C mice and this administration induced the sustained expression of human BLyS and specific IgG against the recombinant BLyS protein. This study shows that recombinant eukaryotic expression plasmid, pcDNA3.1BLyS, might be used in human gene therapy.

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