IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E221

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Detection of airborne trichothecene-producing Fusarium species in chicken houses

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1 College of Food Science Technology, Guangdong Ocean University, Jiefang East Road No.40, Xiashan Zone, Zhanjiang 524088, Guangdong Province, P. R. China
2 College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street No. 61, Taian 271018, Shandong Province, P. R. China
3 College of Life Science, Dalian Nationalities University, Liaohe West Road No. 18, Economic and Technical Development Zone, Dalian 116600, Liaoning Province, P. R. China

*Author to whom correspondence should be addressed.

Academic Editor: Atin Adhikari

Front. Biosci. (Elite Ed) 2011, 3(1), 74–80; https://doi.org/10.2741/E221
Published: 1 January 2011
Abstract

One hundred and forty three airborne Fusarium isolates in chicken houses belonging to seven Fusarium species were analyzed by PCR with Tri5 gene as a specific marker of mycotoxin product . The result of Tri5 gene sequence analysis indicates that the PCR amplification products were 89%-96% identical to the previously reported Tri5 genes, which were all amplified from four F. poae isolates. T-2 toxin and DON was measured by immunoaffinity column and high performance liquid chromatography in Tri5-positive F. poae isolates after being cultured at constant and alternating temperatures. The production of T-2 toxin under alternating temperatures was 14 and 53 times higher than those at constant temperature of 8°C and 25°C. No DON was detected under either testing temperature condition. It is concluded that T-2 toxin-producing F. poae isolates were present in poultry houses, and the concentration of T-2 toxin produced by Tri5-positive F. poae isolates was increased under alternating temperatures. The application of Tri5-PCR associated with IMC-HPLC is an effective and accurate method for rapid detection of T-2 and DON mycotoxins.

Keywords
Fusarium
trichothecene
T-2 toxin
Tri5
PCR
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