IMR Press / FBE / Volume 14 / Issue 2 / DOI: 10.31083/j.fbe1402010
Open Access Original Research
Identification of Major Antioxidant Compounds from the Edible Mushroom Basidiomycetes-X (Echigoshirayukidake)
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1 School of Biological Science and Technology, College of Science and Engineering, Kanazawa University, Kakuma, 920-1192 Kanazawa, Japan
2 Division of Natural System, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, 920-1192 Kanazawa, Japan
3 Kanazawa Kids’ Science Center, Naga-machi, 920-0865 Kanazawa, Japan
4 Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Higashijima, Akiha-ku, 956-8603 Niigata, Japan
5 Office HALD Food Function Research, Inc., Yuzawa, 949-6102 Niigata, Japan
*Correspondence: tsakamot@staff.kanazawa-u.ac.jp (Toshio Sakamoto)
Academic Editor: Baohong Zhang
Front. Biosci. (Elite Ed) 2022, 14(2), 010; https://doi.org/10.31083/j.fbe1402010
Submitted: 26 November 2021 | Revised: 13 January 2022 | Accepted: 17 January 2022 | Published: 6 April 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Basidiomycetes-X, of which Japanese vernacular name is Echigoshirayukidake, is a local speciality mushroom found and cultivated in Japan that has been distributed as a precious cuisine material or as a functional food with medicinal properties. Antioxidant activity-guided isolation of major ingredients in Basidiomycetes-X revealed the presence of ergosterol, trans-10,cis-12-octadecadienoic acid (a conjugated linolenic acid, 10(E),12(Z)-CLA) and 2,3-dihydro-3,5-dihydroxy-6-methyl‑4H‑pyran-4-one (DDMP). Approximately 21% of the 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazino radical (DPPH) scavenging activities in the methanolic extract were related to 10(E),12(Z)-CLA, while approximately 6.2% of the activity was related to ergosterol. DDMP was present in both methanolic and water extracts, and the activity related to DDMP was conspicuously detected in water extracts. Moreover, uridine and adenosine were identified as major components of Basidiomycetes-X. The ingredients identified in Basidiomycetes-X are expected to be involved in biological functions observed in this mushroom, which is an attractive functional food resource.

Keywords
Adenosine Conjugated linoleic acid (CLA) 2,3-dihydro-3,5-dihydroxy-6-methyl‑4H‑pyran-4-one (DDMP) Ergosterol Uridine Radical scavenger
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