IMR Press / FBE / Volume 14 / Issue 1 / DOI: 10.31083/j.fbe1401007
Open Access Original Research
Polyclonal antibodies against potato spindle tuber viroid RNA
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1 Belozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russian Federation
2 Federal Research Centre “Fundamentals of Biotechnology", Russian Academy of Sciences, 119071 Moscow, Russian Federation
3 Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russian Federation
*Correspondence: drygin@belozersky.msu.ru (Yuri Drygin)
Academic Editor: Baohong Zhang
Front. Biosci. (Elite Ed) 2022, 14(1), 007; https://doi.org/10.31083/j.fbe1401007
Submitted: 19 September 2021 | Revised: 10 January 2022 | Accepted: 13 January 2022 | Published: 4 March 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Ribonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with synthesized conjugate viroid RNA-lysozyme. Analyses of polyclonal mouse and rabbit antisera as well as estimates of antibody specificity were performed by dot-Enzyme Linked Immunosorbent Assay (ELISA), sandwich ELISA, and northern immunoblotting. Antiserum obtained showed strong cross-reactions with cellular RNA. The viroid polyclonal antibody cross-reactions with cellular RNAs were depleted via titration antibodies by the plant cellular or commercial yeast RNA. We successfully used antibodies against the viroid RNA-lysozyme antigen to detect the wild-type potato viroid and diagnose potato viroid infection. We presume that intrinsic cross-reactions of RNA antibodies are potentially dangerous after nucleic acid vaccination. Research into the specificity of antibodies against viral RNAs is underway.

Keywords
potato spindle tuber viroid
dot-ELISA
immune northern blotting
diagnostics
nucleic acid vaccine
Figures
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