IMR Press / FBE / Volume 13 / Issue 2 / DOI: 10.52586/E881
Open Access Article
Antimicrobial activity of Lactiplantibacillus plantarum APsulloc 331261 and APsulloc 331266 against pathogenic skin microbiota
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1 AMOREPACIFIC R&D Center, AMOREPACIFIC, 17074 Yongin, Republic of Korea
2 Department of Dermatology, College of Medicine, Chung-Ang University, 06974 Seoul, Republic of Korea
3 Department of Medicine, Graduate School, Chung-Ang University, 06973 Seoul, Republic of Korea
4 Department of Life Science, Sogang University, 04107 Seoul, Republic of Korea

These authors contributed equally.

Front. Biosci. (Elite Ed) 2021, 13(2), 237–248; https://doi.org/10.52586/E881
Submitted: 17 September 2021 | Revised: 19 October 2021 | Accepted: 15 November 2021 | Published: 20 December 2021
Copyright: © 2021 The Author(s). Published by BRI.
This is an open access article under the CC BY 4.0 license (https://creativecommons.org/licenses/by/4.0/).
Abstract

Balanced skin microbiota is crucial for maintaining healthy normal skin function; however, disruption of the balance in skin microbiota is linked with skin diseases such as atopic dermatitis, acne vulgaris, dandruff, and candidiasis. Lactoplantibacillus species with proved with health benefits are probiotics that improve the balance of microbiome in skin and gut. In the present study, we investigated the potential antimicrobial activity of Lactiplantibacillus plantarum APsulloc 331261 (APsulloc 331261) and Lactiplantibacillus plantarum APsulloc 331266 (APsulloc 331266) derived from green tea, in inhibiting five skin pathogenic strains (Staphylococcus aureus (S. aureus), Cutibacterium acnes (C. acnes), Candia albicans (C. albicans), Malassezia globosa (M. globose), and Malassezia restricta (M. restricta)) associated with skin infection. Viability of S. aureus, C. acnes, C. albicans, M. globosa, and M. restricta was inhibited by indirect co-culture with APsulloc 331261 or APsulloc 331266 at various ratios. Different concentrations of the cell-free conditioned media (CM) derived from APsulloc 331261 or APsulloc 331266 inhibited the vaibility of S. aureus, C. acnes, C. albicans, M. globosa and M. restricta in a dose dependent manner. Moreover, susceptibility of S. aureus, C. acnes, and C. albicans against APsulloc 331261 or APsulloc 331266 was confirmed following agar overlay methods. Results of the agar overlay confirmed that various concentrations of APsulloc 331261 and APsulloc 331266 exhibited low to high inhibitory activity on the growth of S. aureus (ZDI 20.3 ± 2.1–32.3 ± 2.1 mm, R value 5.7 ± 0.8–7.8 ± 1.3 mm), C. acnes (ZDI 15.0 ± 1.7–22.2 ± 1.7 mm, R value 3.2 ± 1.3–5.5 ± 1.3 mm) and C. albicans (ZDI 13.3 ± 4.0–27.0 ± 3.6 mm, R value 2.8 ± 1.9–5.5 ± 1.7 mm). Finally, standard PCR analysis identified the presence of the of plantaricin genes encoding antimicrobial peptides in APsulloc 331261 and APsulloc 331266. These results suggest that APsulloc 331261 and APsulloc 331266 has a potential effect in the improvement of the balance of skin microbiota by inhibiting skin pathogenic strains.

Keywords
Lactiplantibacillus plantarum
APsulloc
Green tea
Skin pathogens
Staphylococcus aureus
Cutibacterium acnes
Candia albicans
Malassezia globosa
Malassezia restricta
Plantaricin
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