Background: To evaluate the safety and feasibility of single-handed trans-umbilical single-site laparo-endoscopic surgery using the suspension-line method for salpingectomy in ectopic pregnancy. Methods: This study reviewed 54 patients with ectopic pregnancy who underwent salpingectomy in the First Affiliated Hospital of Xiamen University from June 2018 to June 2019. The control group (n = 29) was treated with routine two-handed trans-umbilical laparoendoscopic single-site surgery (TU-LESS), while the study group (n = 25) was treated with single-handed TU-LESS using the suspension line method. Results: There were no significant differences in clinical indicators such as operation time, postoperative recovery ventilation time, recovery time of \beta-human chorionic gonadotropin (\beta-HCG) to normal level, and postoperative complications (p > 0.05) between two groups, whereas the patients in the study group suffered less pain and were more satisfied with the incision (p 0.05). Conclusions: Single-handed LESS using the suspension method can be safely and effectively applied to salpingectomy, with less trauma, more beautiful incision, and more comfortable operation.