IMR Press / FBL / Volume 27 / Issue 1 / DOI: 10.31083/j.fbl2701008
Open Access Original Research
In vivo and in vitro neuroprotective effects of maca polysaccharide
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1 Hunan Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, 410219 Changsha, Hunan, China
2 Academician Workstation, Changsha Medical University, 410219 Changsha, Hunan, China
3 Department of Orthopedics, Xiangya Hospital Central South University, 410008 Changsha, Hunan, China
*Correspondence: (Yusheng Li); (Chenggong Wang)
These authors contributed equally.
Academic Editor: Marcello Iriti
Front. Biosci. (Landmark Ed) 2022, 27(1), 8;
Submitted: 10 August 2021 | Revised: 20 November 2021 | Accepted: 8 December 2021 | Published: 6 January 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Objective: To explore the protective effect of MP on oxidative damage in vivo and in vitro. Methods: A mouse aging model was induced by intraperitoneal injection of D-galactose (D-gal), and pathological changes in the hippocampal ultrastructure were observed by transmission electron microscopy. The activity of glutathione peroxidase (GSH-Px) and the levels of malondialdehyde (MDA) in brain tissues were evaluated with GSH-Px and MDA assay kits. An MTT assay was used to detect the viability of the model SH-SY5Y cells with H2O2-induced damage, and a lactate dehydrogenase (LDH) kit was used to evaluate LDH leakage. Reactive oxygen species (ROS) levels and cell cycle arrest were analyzed by flow cytometry, and cleaved caspase 3 and P53 protein expression was determined by western blot analysis. Results: Demonstrated that MP increased GSH-Px activity, reduced MDA levels, and attenuated the cell damage induced by H2O2. Furthermore, MP protected neuronal cells from oxidative stress through a mechanism including a decrease in LDH leakage and reversal of H2O2-induced cell morphological damage. MP treatment alleviated the H2O2-induced increases in ROS levels, inhibited apoptosis, relieved cell cycle arrest, and downregulated cleaved caspase 3 and P53 protein expression. Conclusions: MP is a novel antioxidant with neuroprotective effects.

Neuroprotective effect
Reactive oxygen species
SH-SY5Y cells
Fig. 1.
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