Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.
Fabricated solid-state nanopore chips are used to probe individual single stranded DNA (ssDNA) homopolymers. Based on the analysis of the current blockage caused by DNA translocation through a voltage-biased nanopore, we discovered that the hydrodynamic diameter of ssDNA homopolymer helix is comparable to that of double stranded DNA (dsDNA) helix. This proof-of-principle demonstration shows that solid-state nanopore technology can be used to spy on secondary structures of biopolymers. We also show that ssDNA manifests slower and distinct translocation kinetics in comparison to dsDNA. Furthermore, the present study helps to refine our understanding of the ssDNA translocation kinetics through narrower alpha-hemolysin protein pores.