Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.
1 Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland
2 Clinic of Neurology, University of Geneva, Geneva, Switzerland
Abstract
Thanks to deep tissue penetration of infrared light (IR), two-photon laser scanning microscopy (2PLSM) has become an important tool in modern neuroscience. Examples range from the high-resolution visualization of dendritic spines in single neurons to the monitoring of intracellular Ca++ transients in small neural networks. In the present review we give an overview of the current achievements and discuss emerging applications of the technique.
Keywords
- Fluorescence
- GFP
- Multi-photon
- Infrared Light
- Two-Photon Laser Scanning Microscopy
- Neuron
- Synapse
- Network
- Neuroscience
- Imaging
- Calcium
- Fluorophores
- Photobleaching
- Review