IMR Press / JIN / Volume 21 / Issue 6 / DOI: 10.31083/j.jin2106161
Open Access Original Research
Characterization of the Sphingolipidome of the Peri-Infarct Tissue during Hemorrhagic Transformation in a Mouse Model of Cerebral Ischemia
Show Less
1 Department of Neurology, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany
2 Institute of General Pharmacology and Toxicology, Pharmazentrum Frankfurt, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany
3 Institute of Clinical Pharmacology, Pharmazentrum Frankfurt, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany
4 Department of Neuroradiology, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany
5 Department of Neurology, RWTH Aachen University, 52074 Aachen, Germany
6 Department of Medicine, University of Cambridge, CB2 0QQ Cambridge, UK
7 Mildred Scheel Early Career Center, Medical Faculty, Technical University Dresden, 01307 Dresden, Germany
8 Center for Regenerative Therapies, Technical University Dresden, 01307 Dresden, Germany
*Correspondence: (Alexandra Lucaciu); (Julien Subburayalu)
Academic Editor: Beata Sarecka-Hujar
J. Integr. Neurosci. 2022, 21(6), 161;
Submitted: 4 May 2022 | Revised: 3 June 2022 | Accepted: 15 June 2022 | Published: 26 September 2022
(This article belongs to the Special Issue Advances in Acute Ischemic Stroke)
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: Cardiovascular diseases like stroke cause changes to sphingolipid mediators like sphingosine 1-phosphate (S1P) or its ceramide analogs, which bear the potential to either alleviate or exacerbate the neurological damage. Therefore, the precise identification of alterations within the sphingolipidome during ischemic stroke (IS) and hemorrhagic transformation (HT) harbors a putative therapeutic potential to orchestrate local and systemic immunomodulatory processes. Due to the scarcity of research in this field, we aimed to characterize the sphingolipidome in IS and HT. Methods: C57BL/6 mice underwent middle cerebral artery occlusion (MCAO) and specimens of the peri-infarct tissue were taken for sphingolipid profiling. Results: Ischemic stroke resulted in reduced S1P whilst ceramides were elevated six hours post ischemia onset. However, these differences were nearly revoked at 24 hours post ischemia onset. Moreover, the topmost S1P and ceramide levels were linked to the presence of HT after MCAO. In this study we show the characterization of the sphingolipidomic landscape of the peri-infarct tissue after ischemic stroke and HT. Especially, highest values of S1P, C 18 lactosylceramide, C 18 glucosylceramide, and C 24:1 ceramide were nearly entirely expressed by mice with HT. Conclusions: Our results warrant further investigations into the immunomodulatory consequences of altered sphingolipid species for the development of HT after IS.

sphingosine 1-phosphate
S1P 1
ischemic stroke
hemorrhagic transformation
sphingolipid profiling
SFB1039 (ID: 259130777-E02)/German Research Foundation
SFB1039/Z1/German Research Foundation
SFB1039-TPB08/German Research Foundation
Fig. 1.
Back to top