International Journal for Vitamin and Nutrition Research (IJVNR) is published by IMR Press from Volume 95 Issue 1 (2025). Previous articles were published by another publisher under a hybrid publishing model, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Hogrefe.
Pantothenic Acid Quantification: Method Comparison of a Stable Isotope Dilution Assay and a Microbiological Assay
1 Lehrstuhl für Lebensmittelchemie der Technischen Universität München, Lichtenbergstr. 4, D-85748 Garching, Germany
2 Fachgebiet Tierernährung des Departments Tierwissenschaften der Technischen Universität München, Hochfeldweg 6, D-85350 Freising-Weihenstephan, Germany
Abstract
Different foods and feedstuffs were analyzed for pantothenic acid (PA) by the recently developed stable isotope dilution assay (SIDA) and by the standard method, a microbiological assay (MA). The SIDA involved the use of [13C3, 15N]-pantothenic acid as the internal standard and detection by liquid chromatography-tandem mass spectrometry. The analysis of identical extracts minimized systematic bias due to equal extraction yields and enabled an ideal comparison between both methods. For the samples derived from plants a good accordance between the MA and the SIDA of total PA was found, whereas for the products of animal origin, higher contents were measured by MA than by SIDA. From the results of treatments by pantetheinase and phosphatase on the one hand and papain and diastase on the other, it was concluded that MA is able to measure a significant amount of bound PA. Furthermore, the data imply that microbial enzymes were able to cleave PA conjugates more effectively than pantetheinase and phosphatase treatment.
Keywords
- Method comparison
- microbiological assay
- pantothenic acid
- stable isotope dilution assay