Background: Sterile water injection is rapidly gaining popularity as a method of pain relief in labor. The purpose of this single-blinded randomized controlled study is to investigate the effect of intradermal sterile water injection (ISWI) on labor experiences of women who experience low-back pain during labor. Methods: The sample group of the study was 120 pregnant women with severe low-back pain during labor (30-sterile water, 30-saline solution, 60-control). In the study, the baseline low back pain score (>7) was determined with Visual Analogue Scale (VAS) in the ISWI group and then 0.1 mL sterile water made to form 4 small blebs 1 over each posterior superior iliac spine and 2 others placed 3 cm below and 1 cm medial to each of the first sites (Michaelis Rhomboid). The VAS scoring were repeated at 10–30–60–90 and 120th minutes after the injections. In the saline solution group, injection of 0.1 mL saline solution was made with the same procedure. Childbirth Experience Questionnaire (CEQ) were utilized to determine the effect of injections on participants’ labor experiences. Also participants’ satisfaction with the method was evaluated via Intradermal injections assessment form. Results: There was a significant difference between the mean VAS scores in favor of the ISWI group (p 0.05). Also comparisons indicated significant differences in favor of the ISWI group in terms of both CEQ total mean score and women’s thoughts about injections (p 0.05). Conclusions: The findings of this study showed that ISWI was effective in relieving low-back pain during labor and the technique had positive effects on labor experiences.