Academic Editor: Michael H. Dahan
Background: Previous studies shown that RNA binding motif proteins
(RBM) participate in regulating various physiological processes such as cell
autophagy, proliferation, and apoptosis, and are abnormally highly expressed in
placental trophoblast cells intervened by hypoxia in vitro, but their molecular
mechanisms regulating placental trophoblast damage remain unclear. This study
aims to investigate the role and molecular mechanism of RBM10 in regulating
hypoxia-induced placental trophoblast injury through endoplasmic reticulum
stress. Methods: CCK-8 cell proliferation assay and Transwell cell
invasion assay were applied to detect the proliferation and invasion ability of
normal, hypoxic and RBM10 up-regulated plus hypoxic embryonic trophoblast cells,
respectively. The expression of endoplasmic reticulum stress-related proteins
(ERN-1) and C/EBP homologous protein (CHOP), apoptosis-related proteins B cell
lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax) and Caspase-3, and
autophagy-related proteins including microtubule-associated protein 1 light chain
3 (LC-3), Beclin-1 and P62 were also detected by western blot assays. The effects
of hypoxia and overexpression of RBM10 on placental trophoblast apoptosis were
examined using flow cytometry. Results: We found that the growth and
invasion ability of placental trophoblast cells treated with hypoxia were
significantly decreased (p