IMR Press / CEOG / Volume 49 / Issue 11 / DOI: 10.31083/j.ceog4911256
Open Access Original Research
Evidence for Increased Expression of SIRT3 Associated with Hyperandrogenism in Granulosa Cells of Non-Obese PCOS Patients
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1 Reproductive Medicine Center, Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, 400010 Chongqing, China
2 Reproductive Medicine Center, The First Affiliated Hospital, Wenzhou Medical University, 325000 Wenzhou, Zhejiang, China
3 The Fist Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology and Chongqing Eye Institute, 400016 Chongqing, China
4 School of Basic Medical Sciences, Zunyi Medical University, 563003 Zunyi, Guizhou, China
5 Institute of Biomedical Engineering, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), 518020 Shenzhen, Guangdong, China
*Correspondence: (Shen Zhang)
Academic Editor: Miro Šimun Alebić
Clin. Exp. Obstet. Gynecol. 2022, 49(11), 256;
Submitted: 7 May 2022 | Revised: 31 July 2022 | Accepted: 8 August 2022 | Published: 16 November 2022
(This article belongs to the Special Issue Polycystic Ovary Syndrome)
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: SIRT3 regulates the generation of reactive oxygen species (ROS) in human granulosa cells (GCs). Increased levels of oxidative stress may cause follicular dysplasia in GCs of polycystic ovary syndrome (PCOS) patients. However, expression and regulation of SIRT3 in GCs of PCOS patients have not yet been investigated. The present study is conducted to determine the correlation between SIRT3 and hyperandrogenism in luteinized GCs of PCOS patients. Methods: The mRNA and protein expression of SIRT3 were analysed in the luteinized GCs from the controls and non-obese PCOS patients. Dihydrotestosterone (DHT) was added to the primary cultured GCs to test the effects of androgen excess on intracellular ROS and SIRT3 expression. A DHT-induced PCOS murine model was used to confirm the effects in vivo. Results: In the matched case-control study including 32 pairs of the controls and non-obese PCOS patients, we showed that the expression of SIRT3 was increased in luteinized GCs of non-obese PCOS patients compared with normovulatory controls. Moreover, DHT induced oxidative stress and SIRT3 expression in human GCs, which was further confirmed in a murine PCOS model. Conclusions: These results indicated that the increased expression of SIRT3 was induced by hyperandrogenism in GCs of non-obese PCOS patients.

polycystic ovary syndrome
luteinized granulosa cells
oxidative stress
cstc2021jcyj-msxmX0097/Natural Science Foundation of Chongqing Municipality
LQ19H040003/Natural Science Foundation of Zhejiang Province
F-585/Natural Science Foundation of Zunyi Medical University
Fig. 1.
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