IMR Press / FBL / Volume 27 / Issue 10 / DOI: 10.31083/j.fbl2710284
Open Access Original Research
The Potential of Low Phytic Acid1-1 Mutant in Maize (Zea mays L.): A Sustainable Solution to Non-Renewable Phosphorus
Show Less
1 Department of Agricultural and Environmental Sciences—Production Landscape, Agroenergy, Università degli Studi di Milano, 20133 Milan, Italy
2 Food & Environment Research Laboratories—Analytical Chemistry and Technology—Department of Agricultural and Environmental Sciences, University of Milano, 20133 Milan, Italy
3 DBT-North East Centre for Agricultural Biotechnology, Assam Agriculture University, 785013 Jorhat, Assam, India
*Correspondence: (Roberto Pilu)
Academic Editor: Kevin Cianfaglione
Front. Biosci. (Landmark Ed) 2022, 27(10), 284;
Submitted: 20 July 2022 | Revised: 27 September 2022 | Accepted: 27 September 2022 | Published: 14 October 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: Phosphorus is an essential component of fertilizers and feed and in recent decades has become one of the main sustainability issues as a non-renewable resource. In plant seeds, the main reserve of phosphorus is phytic acid, a strong anti-nutritional factor for monogastrics and a pollutant of cultivated lands. The reduction of phytic acid in cereal seeds has become a major challenge in breeding programs to increase the nutritional quality of foods and feeds and to improve the environmental phosphorus sustainability in agriculture. In maize (Zea mays L.), four low phytic acid (lpa) mutations have been isolated and lpa1-1 is the most promising. However, the reduction of phytic acid in lpa1-1 leads to many adverse pleiotropic effects on the seed and in general on plant performance. A seed weight reduction and a consequent yield loss were previously described in this mutant. Method: In this work, a field experiment to study seed weight and yield was conducted for two years in two different genetic backgrounds (B73 and B73/Mo17). Furthermore, the greater susceptibility of lpa1-1 to drought stress was also investigated: a dedicated field experiment was set up and measurements were carried out under optimal water conditions and moderate drought stress. Results: From the first experiment it emerges that under high-input conditions, lpa1-1 seems to have comparable or even better yield than the relative control. The main problem of this mutant remains the reduced field emergence (~40%). In the study of drought stress it was found that the increased sensitivity in the mutant is mainly caused by an altered stomatal regulation, but not by a less developed root system, as previously reported. When the stress occurred, the parameters measured did not significantly change in the wild-type, while they dropped in the mutant: the net photosynthesis decreased by 58%, the transpiration rate by 63% and the stomatal conductance by 67%. Conclusions: Some possible solutions have been proposed, with the aim of developing a commercial variety, which remains the main goal to exploit the nutritional benefits of low phytic acid mutants.

low phytic acid mutants
drought stress
root system architecture
stomatal conductance
environmental sustainability
seed quality
carbon isotope discrimination
Fig. 1.
Back to top