IMR Press / FBL / Volume 2 / Issue 3 / DOI: 10.2741/A165

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

RAPID PURIFICATION OF HISTIDINE-TAGGED GLUTATHIONE S-TRANSFERASE FUSION
PROTEIN BY METAL CHELATE POROS® PERFUSION CHROMATOGRAPHY® MEDIA 

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1 Boehringer Mannheim GmbH, Penzberg, Germany
Front. Biosci. (Landmark Ed) 1997, 2(3), 6–8; https://doi.org/10.2741/A165
Published: 15 February 1997
Abstract

The use of POROS MC media allowed high speed column preparation, sample loading, and elution. Under these conditions the proteins of interest can be recovered under mild conditions to maintain maximum biological activity. The speed of the process also assures minimum degradation or post-extraction modifications of the protein of interest from exposure to naturally occurring proteases and other enzymes. It is evident from the analysis by polyacrylamide gel electrophoresis of the purified fusion protein and starting material that a high degree of purification is possible in a single high-speed separation on POROS MC. The high flow rate capabilities of POROS media offer several other significant advantages over conventional media. A few of these include high speed and systematic method optimization, rapid column preparation and sample loading, and fast time-saving chromatographic separations.

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