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Article
Applications of two-photon microscopy in the neurosciences
Hans G. Cruz1, Christian Lüscher1,2,*
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1
Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland
2
Clinic of Neurology, University of Geneva, Geneva, Switzerland
Front. Biosci. (Landmark Ed) 2005, 10(3), 2263–2278;
https://doi.org/10.2741/1696
Published: 1 September 2005
Abstract
Thanks to deep tissue penetration of infrared light (IR), two-photon laser scanning microscopy (2PLSM) has become an important tool in modern neuroscience. Examples range from the high-resolution visualization of dendritic spines in single neurons to the monitoring of intracellular Ca++ transients in small neural networks. In the present review we give an overview of the current achievements and discuss emerging applications of the technique.
Keywords
Fluorescence
GFP
Multi-photon
Infrared Light
Two-Photon Laser Scanning Microscopy
Neuron
Synapse
Network
Neuroscience
Imaging
Calcium
Fluorophores
Photobleaching
Review