Applications of two-photon microscopy in the neurosciences

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Article

Hans G. Cruz¹, Christian Lüscher^1,2,*

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¹ Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland

² Clinic of Neurology, University of Geneva, Geneva, Switzerland

Academic Editor: Ernst Niggli

Front. Biosci. (Landmark Ed) 2005, 10(3), 2263–2278; https://doi.org/10.2741/1696

Published: 1 September 2005

(This article belongs to the Special Issue Biological applications of multi-photon microscopy)

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Abstract

Thanks to deep tissue penetration of infrared light (IR), two-photon laser scanning microscopy (2PLSM) has become an important tool in modern neuroscience. Examples range from the high-resolution visualization of dendritic spines in single neurons to the monitoring of intracellular Ca⁺⁺ transients in small neural networks. In the present review we give an overview of the current achievements and discuss emerging applications of the technique.

Keywords

Fluorescence

GFP

Multi-photon

Infrared Light

Two-Photon Laser Scanning Microscopy

Neuron

Synapse

Network

Neuroscience

Imaging

Calcium

Fluorophores

Photobleaching

Review

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