Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.
A novel method was used to prepare the nano-composite by assembling nanogold (NG) particles on the multiwall carbon nanotubes (MWNTs) surface. The nano-composite could be immobilized on a glassy carbon (GC) electrode to get a novel modified electrode. The electrode can easily immobilize the horseradish peroxidase (HRP) molecules to construct a reagentless biosensor. The NG particles in the composite film have a good biological compatibility. And due to the existence of quinone groups on the MWNTs surface, the MWNTs can promote the electron transfer between enzymes and electrode surface. The biosensor shows a good stability and responds to H2O2 in the range from 2.0 µM to 3.5 mM with a detection limit of 1.0 µM.