IMR Press / FBL / Volume 10 / Issue 1 / DOI: 10.2741/1535

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Micromechanics of tumor cell adhesion and migration under dynamic flow conditions
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1 Department of Bioengineering, The Pennsylvania State University, University Park, PA 16802-6804, USA

Academic Editor: Cheng Dong

Front. Biosci. (Landmark Ed) 2005, 10(1), 379–384; https://doi.org/10.2741/1535
Published: 1 January 2005
(This article belongs to the Special Issue Cellular biomechanics)
Abstract

Attachment of tumor cells to endothelial cells is critical for migration of tumor cells out of the vascular system to establish metastases. We have studied human melanoma cell (C8161) adhesion and migration in response to stimulation by soluble collagen IV (CIV) using a modified Boyden chamber. In this modified chamber, shear flow can be introduced over the cell-substrate interface affecting tumor cell chemotactic migration through a microporous filter. Our results suggest that transmigration of C8161 cells under flow conditions can be influenced by PMNs, mediated by Mac-1 and ICAM-1 adhesive interactions and enhanced by altered cytokine production.

Keywords
Melanoma
Neutrophil
Shear Stress
Signaling
Extravasation
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